Western Blot Substrates and Substrate Kits
Rockland Immunochemicals Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP - 18-8817-33
Mouse TrueBlot ULTRA has been reported for use in IP and WB. Mouse IgG TrueBlot ULTRA is provided as 1000X solution. To achieve best results when detecting mouse IgG1 subtypes, we recommend performing a dot blot or titration to determine the optimal dilution. Specific conditions for each protein and antibody combination should be specifically optimized by the end user. We recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mLs). If used conservatively at 2.5mls/blot will yield enough reagent for 80 blots. Note that there are three key procedural considerations: 1. Protein A or G beads may be used with the mouse, goat and sheep TrueBlot secondaries, but not with the rabbit TrueBlot secondary. Use of protein A or G beads with the rabbit TrueBlot will result in contaminating bands. 2. Immunoprecipitate should be completely reduced. 3. BLOTTO/Milk should be used as the blocking protein for the immunoblot.
Alkali Scientific BrightStar™ Femto HRP Chemiluminescent Substrate, Pre-Mixed Single Component Luminol and Peroxide, ECL Mix, 100mL
The BrightStar™ Femto HRP Chemiluminescent Substrate is a highly sensitive Luminol-based chemiluminescent substrate that may be used for any blotting applications utilizing horseradish peroxidase (HRP)-conjugated antibodies. The HRP substrates are compatible with various blocking buffers and can be applied on PVDF or nitrocellulose with low backgrounds. The HRP Western substrates come as a single component mix system for added convenience. Visualization may be carried out through x-ray film or a CCD camera. This is a 100mL kit luminol reagent and peroxide solution.
Sigma Aldrich Fine Chemicals Biosciences Atto 488 suitable for fluo1MG
Atto 488 is a labeling dye with high molecular absorption (90,000) and quantum yield (0.80) as well as sufficient Stokes shift between excitation and emission maximum. It is optimized for excitation with an argon laser, and is characterized by high photostability.
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Human & Mouse Total Histone H3 Detection Kit, 500 Assay Points
AlphaLISA SureFire Ultra Human & Mouse Total Histone H3 Detection Kit, 500 Assay Points
Revvity Health Sciences Inc TopSeal-S 96-well, clear, case of 100
TopSeal-S 96-well, clear, case of 100
Revvity Health Sciences Inc Western Lightning Plus, Chemiluminescent Substrate (680 mL)
Western Lightning Plus, Chemiluminescent Substrate (680 mL)
Seracare Life Sciences Inc TMB 1-C MEMBRANE HRP SUBSTRATE
Most sensitive chromogenic substrate for peroxidase detection. It develops a deep blue color at the reaction site in the presence of peroxidase-labeled conjugates. Recommended for membrane assays and dot blots. 1-component format of 3, 3', 5, 5' - Tetramethylbenzidine, no reagent preparation, hence, very convenient.
Seracare Life Sciences Inc LUMIGLO HRP CHEMI SUBS
Non-isotopic, luminol-based chemiluminescent substrate designed for the rapid and sensitive detection of peroxidase labeled-conjugates. It provides increased sensitivity over chromogenic substrates in both blotting and ELISA applications. In blotting applications, permanent results are recorded on X-ray film. The use of LumiGLO allows for multiple stripping and reprobing of blots. In ELISA, positive reactions are read and rapidly detected using a luminometer. LumiGLO provides a dynamic range that is linear for a longer period of time than other chemiluminescent substrates. 2-Component format, simply mix and use. Safe to use: non-hazardous.
Crescent Chemical Co Inc BCIP/NBT READY-TO-USE
BCIP/NBT ready-to-use substrate for immunohistochemistry and blotting Single component substrate solution for detection of alkaline phosphatase in immunohistochemical, in situ hybridization and blotting procedures. A very fine blue-purple precipitate will be localized at sites of
Medchemexpress LLC HY-K1005 500 mL Medchemexpress, Ultra High Sensitive ECL Kit CAS: Purity:>98%
Medchemexpress, HY-K1005 500 mL Ultra High Sensitive ECL Kit CAS: Purity:>98% Medchemexpress has over 10000 novel life-science reagents, reference compounds, APIs and natural compounds for laboratory and scientific use. Other quantity can also be offered.