Western Blot Substrates and Substrate Kits
Rockland Immunochemicals BCIP/NBT MEMBRANE ALKALINE PHOSPHATASE SUBSTRATE - NBT-100
BCIP/NBT Membrane Alkaline Phosphatase Substrate is an artificially manufactured chromogenic substrate for use in sensitive colorimetric assays. Alkaline phosphatase hydrolyzes the BCIP to 5-bromo-4-chloro-3-indole, which is oxidized by oxygen in the atmosphere, yielding a blue dye. In the presence of NBT, an insoluble dark blue precipitate is formed after a reduction reaction instead.
Biolegend Western-Ready™ ECL Substrate Plus Kit
Western-Ready™ ECL Substrate Plus Kit Apps: WB; Size: 200 mL
AdipoGen BCIP p-toluidine salt
Chemical. CAS 6578-06-9. Formula C8H6BrClNO4P . C7H9N. MW 433.62. BCIP is a colorimetric substrate for alkaline phosphatase. Upon enzymatic cleavage by alkaline phosphatase, an indoxyl intermediate is released and subsequently oxidized to produce water-insoluble indigo. It can be used to detect alkaline phosphatase activity by immunohistochemistry IHC or Western blot WB.
Seracare Life Sciences Inc PHOSPHAGLO AP CHEMI SUBSTRATE
PhosphaGLO AP Substrate is the chemiluminescent substrate of choice for easy, reliable detection of picogram protein levels via alkaline-phosphatase (AP) in Western blotting or ELISA. Product is a ready-to-use, 1-component substrate requiring no special blocking solutions. Other features and benefits include: Long glow times up to five days; allows for repeated exposures (Western Blot) or luminosity readings (ELISA). Easy-to-follow protocol; simply add to microwell or blot and detect within minutes. Easily interchangable with other AP chemiluminescent substrates; requires little optimization to switch. Product is stable for two years at 4deg. C; no worries about rapidly degrading substrates. Compatible with film or with a chemiluminescent imager; provides flexibility for recording permanent results. Cost effective; PhosphaGLO offers cost savings per blot when compared to other chemiluminescent AP substrates.
Genhunter Corporation PerfectWestern, Large Rainbow Set of 6 colors
PerfectWestern, Large, Rainbow Set (Qty. 1-4 sets)
Genetex Inc Trident pico Western HRP Substrate
Trident pico Western HRP Substrate can detect horseradish peroxidase at the low pico to mid femtogram level. Trident pico Western HRP Substrate provides superior sensitivity compared to competitor products and is more economical. The substrate is supplied as two components. Substrate may be used for any blotting application utilizing horseradish peroxidase (HRP)-conjugates. The substrate can be used with various blocking buffers and on nitrocellulose or PVDF membranes. Detection and analysis may be done by CCD imaging systems or x-ray film.