Western Blot Substrates and Substrate Kits
Rockland Immunochemicals Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP - 18-8817-33
Mouse TrueBlot ULTRA has been reported for use in IP and WB. Mouse IgG TrueBlot ULTRA is provided as 1000X solution. To achieve best results when detecting mouse IgG1 subtypes, we recommend performing a dot blot or titration to determine the optimal dilution. Specific conditions for each protein and antibody combination should be specifically optimized by the end user. We recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mLs). If used conservatively at 2.5mls/blot will yield enough reagent for 80 blots. Note that there are three key procedural considerations: 1. Protein A or G beads may be used with the mouse, goat and sheep TrueBlot secondaries, but not with the rabbit TrueBlot secondary. Use of protein A or G beads with the rabbit TrueBlot will result in contaminating bands. 2. Immunoprecipitate should be completely reduced. 3. BLOTTO/Milk should be used as the blocking protein for the immunoblot.
Genhunter Corporation PerfectWestern, Large Rainbow Set of 6 colors
PerfectWestern, Large, Rainbow Set (Qty. 1-4 sets)
Seracare Life Sciences Inc LUMIGLO RES HRP CHEMI SUBS
LumiGLO Reserve is a lumino-based chemiluminescent substrate designed for rapid and sensitive detection of peroxidase-labeled conjugates. This substrate provides maximum sensitivity in the femtogram range, making it ideal for applications where the protein of interest is in low concentrations. Given the high light intensity, images may be more quickly captured and multiple exposures easily obtained. The 2-component format is easily prepared and is provided with KPL's Wash Solution, futher enabling blots with excellent signal-to-noise.
Seracare Life Sciences Inc PHOSPHAGLORESERVE AP SUBSTRATE
PhosphaGLO Reserve AP Substrate is the chemiluminescent substrate of choice when greatest senstivity is required for detection of alkaline-phosphatase (AP) in Western blotting or ELISA. Product is a ready-to-use, 1-component substrate requiring no special blocking solutions. Other features and benefits include: Excellent sensitivity; detect femotogram levels of protein. Long glow times up to five days; allows for repeated exposures (Western Blot) or luminosity readings (ELISA). Easy-to-follow protocol; simply add to microwell or blot and detect within minutes. Product is stable for two years at 4deg. C; no worries about rapidly degrading substrates. Compatible with film or chemiluminescent imager; provides flexibility for recording permanent results.
FUJIFILM BIOSCIENCES INC SeeDB2 Trial Kit
SeeDB2 Trial Kit is a tissue clearing reagent kit for SeeDB2 method This method was developed by Dr Meng-Tsen Ke and Dr Takeshi Imai etc This kit is constituted saponin SeeDB2S Solution SeeDB2G Solution and PBS A block of brain tissue can be made transparent by only soaking into solution in the prepared kit The method can preserve fine structure of the brain tissue and adjust refractive index 1 52 suitable for oil immersion lens used high resolution imaging Its available for a quantitative analysis of dendrite spine
Alkali Scientific BrightStar™ Femto HRP Chemiluminescent Substrate, Pre-Mixed Single Component Luminol and Peroxide, ECL Mix, 100mL
The BrightStar™ Femto HRP Chemiluminescent Substrate is a highly sensitive Luminol-based chemiluminescent substrate that may be used for any blotting applications utilizing horseradish peroxidase (HRP)-conjugated antibodies. The HRP substrates are compatible with various blocking buffers and can be applied on PVDF or nitrocellulose with low backgrounds. The HRP Western substrates come as a single component mix system for added convenience. Visualization may be carried out through x-ray film or a CCD camera. This is a 100mL kit luminol reagent and peroxide solution.
National Diagnostics ProtoGlow ECL | 200mL
ProtoGlow ECL delivers the latest technology developed for enhanced chemiluminescent detection on Western blots. The unique chemistry of the ProtoGlow ECL system increases the sensitivity of Western blots by up to 20 fold. This allows the detection of proteins at lower abundance and/or the use of higher dilutions of primary and secondary antibodies, economizing on these expensive reagents.
Sigma Aldrich Fine Chemicals Biosciences Atto 488-Biotin BioReagent1MG
REAGENT Atto 488-Biotin BioReagent1MG
Abcam ECL Western Blotting Substrate Kit, 50 Tests
The ECL Western Blotting Substrate is a highly sensitive, nonradioactive, enhanced luminol-based chemiluminescent substrate for easy detection of horseradish peroxidase (HRP) on immunoblots.
The product is subject to the following: Abcam Restricted Use Statement